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89117
PhosphoPlus® eIF2α (Ser51) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® eIF2α (Ser51) Antibody Duet #89117

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PhosphoPlus® eIF2α (Ser51) Antibody Duet: Image 1
Western blot analysis of extracts from C2C12 cells, untreated or thapsigargin-treated, using Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb (upper) or eIF2α Antibody #9722 (lower).
PhosphoPlus® eIF2α (Ser51) Antibody Duet: Image 2
Western blot analysis of extracts from various cell lines using eIF2α (D7D3) XP® Rabbit mAb.
PhosphoPlus® eIF2α (Ser51) Antibody Duet: Image 3
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, untreated (left) or λ phosphatase-treated (right), using Phopsho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb.
PhosphoPlus® eIF2α (Ser51) Antibody Duet: Image 4
Immunoprecipitation/western blot analysis of lysates from HeLa cells. Lane 1 contains lysate input (10%), lane 2 was immunoprecipitated with non-specific rabbit IgG, lane 3 was immunoprecipitated with eIF2α (D7D3) XP® Rabbit mAb #5324. Western blot analysis was performed using eIF2α (L57A5) Mouse mAb #2103.
PhosphoPlus® eIF2α (Ser51) Antibody Duet: Image 5
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb.
PhosphoPlus® eIF2α (Ser51) Antibody Duet: Image 6
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using eIF2α (D7D3) XP® Rabbit mAb.
PhosphoPlus® eIF2α (Ser51) Antibody Duet: Image 7
Immunohistochemical analysis of paraffin-embedded human lymphoma using Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb.
PhosphoPlus® eIF2α (Ser51) Antibody Duet: Image 8
Immunohistochemical analysis of paraffin-embedded mouse colon using eIF2α (D7D3) XP® Rabbit mAb.
To Purchase # 89117
Cat. # Size Qty. Price
89117S
1 Kit $ 574

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).
  1. Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
  2. de Haro, C. et al. (1996) FASEB J. 10, 1378-87.
  3. Kaufman, R.J. (1999) Genes Dev. 13, 1211-33.
  4. Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-8.
  5. Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-6.
  6. Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-90.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
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