eIF4A2 (F3S3X) Rabbit mAb #41385
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 48 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
eIF4A members (eIF4A1/2/3) are highly homologous proteins with non-redundant functions. eIF4A1 and eIF4A2 function within the cytoplasm, and their expression is correlated, whereas eIF4A3 functions in the nucleus as part of the exon junction complex (EJC) (6,7). eIF4A2 activity is critical in the functional gene regulation of microRNAs (8). eIF4A2 is capable of inhibiting deadenylation of mRNAs via CNOT7 inhibition (9). During stress response, eIF4A2 is sequestered into granules through Lys226 sumoylation (10). eIF4A2 plays an active role in promoting viral life cycle (11,12). Higher expression of eIF4A2 has been shown in different cancer types including esophageal squamous cell carcinoma and colorectal cancer (13,14).
- Rogers, G.W. et al. (2001) J. Biol. Chem. 276, 12598-12608.
- Rogers, G.W. et al. (1999) J. Biol. Chem. 274, 12236-12244.
- Svitkin, Y.V. et al. (2001) RNA 7, 382-394.
- Li, J. and Li, W.X. (2006) Nat. Cell Biol. 8, 1407-1414.
- Affolter, M. and Pyrowolakis, G. (2006) Nat. Cell Biol. 8, 1319-1321.
- Lu, W.T. et al. (2014) Biochem Soc Trans 42, 166-72.
- Galicia-Vázquez, G. et al. (2012) RNA 18, 1373-84.
- Meijer, H.A. et al. (2013) Science 340, 82-5.
- Meijer, H.A. et al. (2019) Nucleic Acids Res 47, 8224-8238.
- Jongjitwimol, J. et al. (2016) J Cell Sci 129, 2407-15.
- Ndzinu, J.K. et al. (2018) Microbes Infect 20, 346-352.
- Song, Z. et al. (2019) Res Vet Sci 123, 39-46.
- Chen, Z.H. et al. (2019) J Exp Clin Cancer Res 38, 196.
- Lyu, S. et al. (2020) Oncol Lett 20, 177.
Limited Uses
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