Render Target: STATIC
Render Timestamp: 2024-12-09T10:43:22.031Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-09-30 01:54:08.540
Product last modified at: 2024-12-04T10:15:15.139Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ELMO1 (D4K2E) Rabbit mAb #14457

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ELMO1 (D4K2E) Rabbit mAb recognizes endogenous levels of total ELMO1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala567 of human ELMO1 protein.

    Background

    Engulfment and cell motility 1 (ELMO1) is a cell motility and migration protein that interacts with DOCK180 to form an atypical, two-part guanine nucleotide exchange factor (GEF) for the small GTPase Rac (1). The resultant localized Rac activation allows actin nucleation via WAVE family proteins, signaling to integrins, formation of lamellipodia and filopodia, and regulation of processes including phagocytosis and cell migration (2-4). Research studies indicate that DOCK180 and ELMO1 regulate cell migration in lymphocytes (5) and in ovarian cancer cells (6). ELMO1 also promotes Rac1-dependent cell motility through its interaction with the adaptor protein Nck-1 (7), and binds Arhgef16 to promote RhoG/Rac1-dependent engulfment of apoptotic cells by phagocytes (8). Polymorphisms in the corresponding ELMO1 gene may be associated with susceptibility to diabetic neuropathy seen in selected populations of type II diabetic patients (9,10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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