Eosinophil cationic protein (F3G7N) Rabbit mAb #99106

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Supporting Data

REACTIVITY	M
SENSITIVITY	Endogenous
MW (kDa)	18
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Eosinophil cationic protein (F3G7N) Rabbit mAb recognizes endogenous levels of total Eosinophil cationic protein. By overexpression, this antibody cross-reacts with mouse Eosinophil cationic-type ribonuclease 3/Ear3 protein but not Eosinophil cationic protein 2/Ear2, Eosinophil-associated ribonuclease 10/Ear10, or Eosinophil cationic-type ribonuclease 5/Ear5 proteins. This antibody detects bands of unknown origin at 50 and 70 kDa in some cell lines and tissues. Non-specific staining was observed in crypts of mouse stomach and small intestine by immunohistochemistry.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro30 of mouse Eosinophil cationic protein.

Background

Eosinophil cationic protein (ECP, RNASE3) is a member of the ribonuclease superfamily which has both cytotoxic and noncytotoxic properties (1-4). ECP is primarily produced and stored in the secondary granules of eosinophils, where it is involved in host defense against parasites, bacteria, and viruses (1-4). It has also been detected in the primary granules of circulating neutrophils, and can be synthesized by stimulated neutrophils (3,4). ECP is often used as a marker for eosinophilic disease and is measurable in samples such as serum, bronchoalveolar lavage, and nasal secretions (4). A large number of clinical studies are focused on the role of ECP in eosinophil-related disorders, particularly in asthma (5).

Database Links

UniProt ID: P97426

Entrez-Gene Id: 13586

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