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46403
EpCAM (VU1D9) Mouse mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

EpCAM (VU1D9) Mouse mAb (BSA and Azide Free) #46403

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Filter:
  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: EpCAM (VU1D9) Mouse mAb (BSA and Azide Free)
Western blot analysis of extracts from HT-29 and HCT-15 cells using EpCAM (VU1D9) Mouse mAb. Data was generated using the standard formulation of this product.
Immunohistochemistry Image 1: EpCAM (VU1D9) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using EpCAM (VU1D9) Mouse mAb. Data was generated using the standard formulation of this product.
Immunohistochemistry Image 2: EpCAM (VU1D9) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon using EpCAM (VU1D9) Mouse mAb. Data was generated using the standard formulation of this product.
Immunofluorescence Image 1: EpCAM (VU1D9) Mouse mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of HT-29 cells using EpCAM (VU1D9) Mouse mAb (green), Alexa Fluor® 555 Phalloidin #8953 (red), and DRAQ5® #4084 (fluorescent DNA dye). Data was generated using the standard formulation of this product.
To Purchase # 46403
Cat. # Size Qty. Price
46403SF
100 µg $ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 40
Source/Isotype Mouse IgG1

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #2929. All data was generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #2929

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

EpCAM (VU1D9) Mouse mAb (BSA and Azide Free) detects endogenous levels of total EpCAM protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with NCI-H69 small cell lung carcinoma cells.

Background

Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue. Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3).
  1. Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
  2. Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
  3. Armstrong, A. and Eck, S.L. (2003) Cancer Biol Ther 2, 320-6.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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