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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

EphB2 Antibody recognizes endogenous levels of total EphB2 protein. This antibody also cross-reacts with an unidentified protein of 85 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser575 of human EphB2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

The ephrin receptor B2 (EphB2) is an ephrin family receptor tyrosine kinase that plays an important role in regulating growth and development of multiple tissues and organs (1,2). The EphB2 transmembrane receptor protein contains a kinase domain, a PDZ motif, and a SAM domain within a conserved cytoplasmic domain. A ligand binding domain, a cysteine-rich domain, and fibronectin type III repeats comprise the conserved EphB2 extracellular domain (3). EphB2 binds with high affinity to ephrin B ligands, and to some ephrin A proteins, to initiate bidirectional signaling between neighboring cells (1,2). Upon binding, EphB2-Ephrin B2 dimers form a heterotetramer and position the receptor-ligand complex on the cell membrane to facilitate bidirectional signal transduction (3). In addition to associating with ephrin ligands, EphB2 also regulates a number of biological processes through interaction with focal adhesion kinase (FAK), NMDA receptor (NMDAR), the Rac1 guanine nucleotide exchange factor Tiam1, and p21-activated kinase (PAK1) (4-7). While some studies support a role for EphB2 as a pro-oncogenic kinase, other research suggests that EphB2 acts as a tumor suppressor (1,2,4,8).

1.  Pasquale, E.B. (2008) Cell 133, 38-52.

2.  Klein, R. (2009) Nat Neurosci 12, 15-20.

3.  Himanen, J.P. et al. (2001) Nature 414, 933-8.

4.  Wang, S.D. et al. (2012) Oncogene 31, 5132-43.

5.  Nolt, M.J. et al. (2011) J Neurosci 31, 5353-64.

6.  Tolias, K.F. et al. (2007) Proc Natl Acad Sci U S A 104, 7265-70.

7.  Srivastava, N. et al. (2013) Mol Cell Neurosci 52, 106-16.

8.  Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.

Entrez-Gene Id 2048
Swiss-Prot Acc. P29323

For Research Use Only. Not For Use In Diagnostic Procedures.
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EphB2 Antibody