ERCC1 (D6G6) XP® Rabbit mAb (BSA and Azide Free) #31223
- WB
- IHC
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 39 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
ERCC1 (D6G6) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total ERCC1 isoforms 1, 2 and 4 protein. Experiments utilizing overexpression constructs indicated that this antibody does not cross-react with ERCC1 isoform 3.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ERCC1 protein.
Background
DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals (1). Excision Repair Cross Complementing 1 (ERCC1) forms a complex with ERCC4/XPF, which acts as the 5’ endonuclease required to excise the lesion (2). ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) (3) and involved in repair of double strand breaks (4). Research studies have shown that expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (NSCLC) (5,6).
- Shuck, S.C. et al. (2008) Cell Res 18, 64-72.
- McDaniel, L.D. and Schultz, R.A. (2008) Adv Exp Med Biol 637, 65-82.
- Niedernhofer, L.J. et al. (2004) Mol Cell Biol 24, 5776-87.
- Ahmad, A. et al. (2008) Mol Cell Biol 28, 5082-92.
- Zheng, Z. et al. (2007) N Engl J Med 356, 800-8.
- Gossage, L. and Madhusudan, S. (2007) Cancer Treat Rev 33, 565-77.
Limited Uses
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