Erk5 (D3I5V) Rabbit mAb #12950
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 115 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Erk5 (D3I5V) Rabbit mAb recognizes endogenous levels of total Erk5 protein. This antibody detects isoforms 1 and 2 of human Erk5 and isoforms 1-3 of murine Erk5 (note multiple bands in western blot image).
Species Reactivity:
Human, Mouse, Rat, Monkey
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Horse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu556 of human Erk5 protein.
Background
Erk5 (Mitogen-activated protein kinase 7, Big mitogen-activated protein kinase 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation, and survival (1-4). Like other MAPK family members, Erk5 contains a canonical activation loop TEY motif (Thr218/Tyr220) that is specifically phosphorylated by MAP2K5 (MEK5) in a growth-factor-dependent, Ras-independent mechanism (5-7). For example, EGF stimulation promotes Erk5 phosphorylation that induces its translocation to the nucleus where it phosphorylates MEF2C and other transcriptional targets (5,6). Erk5 is also activated in response to granulocyte colony-stimulating factor (G-CSF) in hematopoietic progenitor cells where it promotes survival and proliferation (7). In neuronal cells, Erk5 is required for NGF-induced neurite outgrowth, neuronal homeostasis, and survival (8,9). Erk5 is thought to play a role in blood vessel integrity via maintenance of endothelial cell migration and barrier function (10-12). Although broadly expressed, research studies have shown that mice lacking erk5 display numerous cardiac defects, suggesting Erk5 plays a critical role in vascular development and homeostasis (1,2).
- Zhou, G. et al. (1995) J Biol Chem 270, 12665-9.
- Hayashi, M. and Lee, J.D. (2004) J Mol Med 82, 800-8.
- Wang, X. and Tournier, C. (2006) Cell Signal 18, 753-60.
- Nishimoto, S. and Nishida, E. (2006) EMBO Rep 7, 782-6.
- Kato, Y. et al. (1998) Nature 395, 713-6.
- Kato, Y. et al. (1997) EMBO J 16, 7054-66.
- Dong, F. et al. (2001) J Biol Chem 276, 10811-6.
- Obara, Y. et al. (2009) J Biol Chem 284, 23564-73.
- Finegan, K.G. et al. (2009) Cell Death Differ 16, 674-83.
- Spiering, D. et al. (2009) J Biol Chem 284, 24972-80.
- Sawhney, R.S. et al. (2009) J Cell Physiol 219, 152-61.
- Zhao, Z. et al. (2009) Mol Cell Biochem 322, 171-8.
Limited Uses
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