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ESAM (F2Y5Q) Rabbit mAb #41302

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  • WB
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 40-65
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:300 - 1:1200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ESAM (F2Y5Q) Rabbit mAb recognizes endogenous levels of total ESAM protein. This antibody detects a 75 kDa protein of unknown identity in some cell lines. Non-specific staining was observed in limited peripheral nerves, and in cardiac and skeletal muscle by immunohistochemistry.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp351 of human ESAM protein.

    Background

    Endothelial cell-selective adhesion molecule (ESAM) is an immunoglobulin-like transmembrane protein expressed by endothelial cells and activated platelets (1-4). ESAM is associated with supporting the structural integrity of endothelial cell tight junctions (1-4). ESAM also plays a role in immune responses, aiding in the adhesion of leukocytes to the endothelial cells of blood vessels (4). In mice, expression levels of ESAM are used to differentiate subsets of dendritic cells (5,6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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