View Featured Offers >>
Evi-1 (C50E12) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

Evi-1 (C50E12) Rabbit mAb (BSA and Azide Free) #69659

Reviews ()
Citations (0)
  1. WB
  2. IF
  3. F
Western Blotting Image 1: Evi-1 (C50E12) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using Evi-1 (C50E12) Rabbit mAb. MDS/Evi-1 denotes the fusion between MDS1 and Evi-1 (see reference #6) and Evi-1 Δ denotes the truncated form of Evi-1 (see reference #5). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: Evi-1 (C50E12) Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of SK-OV-3 (left) and DLD-1 cells (right) using Evi-1 (C50E12) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Data were generated using the standard formulation of this product.
Flow Cytometry Image 1: Evi-1 (C50E12) Rabbit mAb (BSA and Azide Free)
Flow cytometric analysis of Jurkat cells (blue) and K562 cells (green) using Evi-1 (C50E12) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.
To Purchase # 69659
Cat. # Size Qty. Price
100 µg
$ 758

Supporting Data

MW (kDa) 105, 145, 200
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&T-CUT&Tag
  • DB-Dot Blot
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #2593. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.


Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #2593.


Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Evi-1 (C50E12) Rabbit mAb (BSA and Azide Free) detects the endogenous levels of total Evi-1 protein.

Species Reactivity:


Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu56 of human Evi-1.


Evi-1 (Ecotropic virus integration site 1) was originally identified as a common site of viral integration in murine myeloid leukemia. It is involved in human myeloid disorders through chromosome translocation and inversion (1) and is also implicated in solid tumor formation (2). Evi-1 is a zinc finger transcription factor which also plays an important role in animal development (3). It has many isoforms due to alternative usage of 5'-ends (4), alternative splicing (5), and intergenic splicing which results in the formation of a fusion protein with MDS1 in normal tissues (6).
  1. Nucifora, G. et al. (2006) Gene 368, 1-11.
  2. Brooks, D.J. et al. (1996) Br. J. Cancer 74, 1518-25.
  3. Hoyt, P.R. et al. (1997) Mech. Dev. 65, 55-70.
  4. Aytekin, M. et al. (2005) Gene 356, 160-8.
  5. Morishita, K. et al. (1990) Oncogene 5, 963-71.
  6. Fears, S. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 1642-7.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.