Render Target: STATIC
Render Timestamp: 2024-12-11T12:17:32.455Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:59:12.480
Product last modified at: 2024-11-27T22:00:09.041Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

EXO1 (E6P2B) Rabbit mAb #63862

Filter:
  • WB

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 120
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    EXO1 (E6P2B) Rabbit mAb recognizes endogenous levels of total EXO1 protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro377 of human EXO1 protein.

    Background

    Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-γ), PMS2 (MutL-α), or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases, and DNA ligases (1). In addition to its role in MMR, the nuclease EXO1 functions in DNA double-strand break repair, replication stress response, nucleotide excision repair (NER), and telomere maintenance (2). AMPK phosphorylates EXO1 at Ser746 in response to replication stress and subsequent calcium elevation to avoid replication fork resection (3). Mutations in the EXO1 gene are associated with multiple human cancers (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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