Interested in promotions? | Click here >>
25514
F4/80 (D2S9R) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

F4/80 (D2S9R) XP® Rabbit mAb (BSA and Azide Free) #25514

Reviews ()
Citations (0)
Filter:
  1. WB
  2. IHC
Western Blotting Image 1: F4/80 (D2S9R) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using F4/80 (D2S9R) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data was generated using the standard formulation of this product.
Immunohistochemistry Image 1: F4/80 (D2S9R) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse spleen using F4/80 (D2S9R) XP® Rabbit mAb. Data was generated using the standard formulation of this product.
Immunohistochemistry Image 2: F4/80 (D2S9R) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse small intestine using F4/80 (D2S9R) XP® Rabbit mAb. Data was generated using the standard formulation of this product.
Immunohistochemistry Image 3: F4/80 (D2S9R) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse liver using F4/80 (D2S9R) XP® Rabbit mAb. Data was generated using the standard formulation of this product.
Immunohistochemistry Image 4: F4/80 (D2S9R) XP® Rabbit mAb (BSA and Azide Free)
Multiplex immunohistochemical analysis of paraffin-embedded mouse LL2 syngeneic tumor tissue using F4/80 (D2S9R) XP® rabbit mAb (orange), PD-1 (D7D5W) XP® rabbit mAb #84651 (green), PD-L1 (D5V3B) rabbit mAb #64988 (yellow), CD3ε (D4V8L) rabbit mAb #99940 (red), CD8α (D4W2Z) XP® rabbit mAb #98941 (magenta) and pan keratin (C11) mouse mAb #4279 (cyan). Data was generated using the standard formulation of this product.
Immunohistochemistry Image 5: F4/80 (D2S9R) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Raw 264.7 cell pellet (left, positive) or Neuro-2A cell pellet (right, negative) using F4/80 (D2S9R) XP® Rabbit mAb. Data was generated using the standard formulation of this product.
To Purchase # 25514
Cat. # Size Qty. Price
25514SF
100 µg $ 746

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 65-250
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #70076. All data was generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #70076

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

F4/80 (D2S9R) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total F4/80 protein.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant mouse F4/80 protein.

Background

F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (1-3). Expression of F4/80 has also been observed in microglia and subset populations of dendritic cells (4).
  1. Hirsch, S. et al. (1981) J Exp Med 154, 713-25.
  2. Austyn, J.M. and Gordon, S. (1981) Eur J Immunol 11, 805-15.
  3. McKnight, A.J. et al. (1996) J Biol Chem 271, 486-9.
  4. Greter, M. et al. (2015) Front Immunol 6, 249.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.