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60557
FAK (D5O7U) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

FAK (D5O7U) XP® Rabbit mAb (BSA and Azide Free) #60557

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: FAK (D5O7U) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using FAK (D5O7U) XP® Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 2: FAK (D5O7U) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from control 293 cells (lane 1), or FAK knockout 293 cells (lane 2) using FAK (D5O7U) XP® Rabbit mAb (upper) or #8457 β-Actin (D6A8) Rabbit mAb (lower). The absence of signal in FAK knockout 293 cells confirms specificity of the antibody for FAK. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: FAK (D5O7U) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using FAK (D5O7U) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: FAK (D5O7U) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using FAK (D5O7U) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: FAK (D5O7U) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using FAK (D5O7U) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: FAK (D5O7U) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using FAK (D5O7U) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: FAK (D5O7U) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using FAK (D5O7U) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: FAK (D5O7U) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded HT-29 cell pellet (left, positive) and RPMI 8226 cell pellet (right, negative) using FAK (D5O7U) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: FAK (D5O7U) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using FAK (D5O7U) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: FAK (D5O7U) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of OVCAR3 cells (left, high-expressing) and SK-OV-3 cells (right, moderate-expressing) using FAK (D5O7U) XP® Rabbit mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087. Data were generated using the standard formulation of this product.
To Purchase # 60557
Cat. # Size Qty. Price
60557SF
100 µg
$ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 125
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier-free version of product #71433. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #71433.

Storage

Store at –20°C. This product will freeze at –20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

FAK (D5O7U) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total FAK protein. Immunofluorescence was performed on cells plated on tissue culture grade plastic. We did not detect FAK signal on uncoated glass slides.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val793 of human FAK protein.

Background

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).
  1. Parsons, J.T. et al. (2000) Oncogene 19, 5606-13.
  2. Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8.
  3. Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55.
  4. Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34.
  5. Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6.
  6. Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63.
  7. Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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