FAR1 (F2Z9W) Rabbit mAb #38678

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Supporting Data

REACTIVITY	H R
SENSITIVITY	Endogenous
MW (kDa)	52
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

FAR1 (F2Z9W) Rabbit mAb recognizes endogenous levels of total FAR1 protein. This antibody does not cross-react with FAR2 protein.

Species Reactivity:

Human, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro52 of human FAR1 protein.

Background

Genome-wide CRISPR-Cas9 suppressor screens show that peroxisomes contribute to ferroptosis in human renal and ovarian carcinoma cells by synthesizing polyunsaturated ether phospholipids (PUFA-ePLs). PUFA-ePLs serve as substrates for lipid peroxidation. Genes identified in the screens include those for alkylglycerone phosphate synthase (AGPS) and fatty acyl-CoA reductase 1 (FAR1), two peroxisomal enzymes catalyzing ether lipid biosynthesis. Studies suggest that AGPS and FAR1 contribute to ferroptosis sensitivity (1,2). In addition, research studies show that FAR1 is essential for ferroptosis mediated by long-chain saturated fatty acids. The expression levels of FAR1 and ferroptosis response are positively correlated in various cancer cell lines (3).

Database Links

UniProt ID: Q8WVX9

Entrez-Gene Id: 84188

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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