FE65 (E4Y6C) Rabbit mAb #29663
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
FE65 (E4Y6C) Rabbit mAb recognizes endogenous levels of total FE65 protein. This antibody may detect non-specific bands of unknown origin at 19 kDa.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro246 of human FE65 protein.
Background
FE65, FE65L1, and FE65L2 are members of the FE65 protein family. FE65 is an adaptor protein with protein-protein interaction domains, including a WW domain followed by two phosphotyrosine interaction domains (PID1 and PID2) (1). Amyloid-β (Aβ) precursor protein (APP) binds to PID2 and undergoes sequential cleavage. First, α-/β secretases cleave and release the ectodomain into the extracellular environment. Subsequent processing by the γ-secretase complex results in the APP intracellular domain (AICD) and the Aβ peptides. The latter Aβ fragments form the main components of amyloid plaques in patients with Alzheimer's disease (2). FE65 family members can regulate APP processing, resulting in elevated levels of Aβ (3). Double knockout mice of FE65 and FE65L1 display a phenotype that occurs in animals lacking APP family members, supporting a functional interaction between FE65 and APP (4).
Limited Uses
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