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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

FGL1 (E8V9Q) Rabbit mAb #47507

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  • WB
  • IF
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 32
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800
    Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    FGL1 (E8V9Q) Rabbit mAb recognizes endogenous levels of total FGL1 protein. This antibody detects a faint cross-reactive band around 27 kDa of unknown identity in some cell extracts.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant human FGL1 protein.

    Background

    FGL1 (fibrinogen-like protein 1, also known as hepassocin) is a secreted protein mainly expressed in liver and its expression is further enhanced following acute liver injury (1-3). FGL1 mice have multiple metabolic abnormalities and are more prone to develop hepatocellular carcinoma under certain experimental conditions, suggesting FGL1 has diverse functions in vivo (4,5). More recently, FGL1 was found to be highly expressed in human cancer cells and function as an inhibitory ligand for LAG3. Blockade of the FGL1-LAG3 interaction stimulates tumor immunity in mouse models. In addition, a high plasma FGL1 level in human cancer patients is associated with a poor prognosis and resistance to anti-PD-1/PD-L1 immunotherapy (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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