Render Target: STATIC
Render Timestamp: 2024-11-01T10:10:02.532Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-08-01 15:28:43.267
Product last modified at: 2024-10-01T14:15:08.764Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Fgr Antibody #2755

Filter:
  • WB
  • IP
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 56
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Flow Cytometry (Fixed/Permeabilized) 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Fgr Antibody detects endogenous levels of total Fgr proteins. This antibody does not cross-react with other proteins.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues close to the amino terminus of human Fgr. Antibodies are purified by protein A and peptide affinity chromatography.  

    Background

    Fgr (c-Fgr kinase) is a member of the Src tyrosine kinase family. It has a membrane-associated amino-terminal domain that is highly divergent from other family members, internal conserved SH2 and SH3 domains, and a highly conserved carboxy-terminal tyrosine kinase catalytic domain (1,2). Tyrosine 412 is located in the activation loop; autophosphorylation of this residue is critical for the activation of Fgr tyrosine kinase activity, and is counter-regulated by Src-mediated phosphorylation at Tyr523 (3). c-Fgr is predominantly expressed in cells of hematopoietic origin, including differentiated myeloid cells, NK, and B cells (4,5). Fgr plays an important role in the signaling cascade from membrane receptors lacking intrinsic tyrosine kinase activity, such as Bcr, FcR, and the integrin family of receptors (6). It was demonstrated that Fgr functions as a selective inhibitor of beta 2 integrin-mediated signaling and Syk kinase function in monocytes (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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