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44800
Fibronectin/FN1 (E5H6X) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

Fibronectin/FN1 (E5H6X) Rabbit mAb (BSA and Azide Free) #44800

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Filter:
  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: Fibronectin/FN1 (E5H6X) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using Fibronectin/FN1 (E5H6X) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The absence of Fibronectin/FN1 expression in PANC-1 cell extracts is consistent with molecular and proteomic expression profiling data, confirming specificity of the antibody. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Fibronectin/FN1 (E5H6X) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Fibronectin/FN1 (E5H6X) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Fibronectin/FN1 (E5H6X) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: Fibronectin/FN1 (E5H6X) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using Fibronectin/FN1 (E5H6X) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: Fibronectin/FN1 (E5H6X) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human adenoid cystic carcinoma of the trachea using Fibronectin/FN1 (E5H6X) Rabbit mAb (left) or Fibronectin Rabbit mAb (right). These two antibodies detect independent, unique epitopes on human Fibronectin/FN1 protein. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: Fibronectin/FN1 (E5H6X) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: Fibronectin/FN1 (E5H6X) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Hep G2 cell pellet (left, positive) or PANC-1 cell pellet (right, negative) using Fibronectin/FN1 (E5H6X) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: Fibronectin/FN1 (E5H6X) Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of U-118 MG cells (left, positive) and PANC-1 cells (right, negative) using Fibronectin/FN1 (E5H6X) Rabbit mAb (green). Actin filaments were labeled with DyLight 650 Phalloidin #12956 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
To Purchase # 44800
Cat. # Size Qty. Price
44800SF
100 µg $ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 300
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #26836. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #26836

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Fibronectin/FN1 (E5H6X) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total Fibronectin/FN1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a protein fragment corresponding to residues near the carboxy terminus of human Fibronectin/FN1 protein.

Background

Fibronectin is a multi-domain extracellular matrix (ECM) protein. The protein uses its different domains to bind distinct ECM components such as collagens, growth factors, as well as cell surface integrins to carry on its functions (1). Fibronectin has been implicated in many essential biological processes including tissue repair, fibrosis, and tumor development (1,2). There are many fibronectin isoforms. Plasma fibronectin is synthesized by hepatocytes and exists as a compact, inactive conformation in the blood stream. It is a major component of fibrin clots. Upon binding to integrins or other cell surface receptors, fibronectin switches to an extended conformation, and exposes its function domains to activated extracellular matrix assembly (3). Fibroblast, endothelial cells, as well as many types of cancer cells have also been shown to synthesize cellular fibronectin isoforms. Among them, the EDA or EDB isoforms are particularly highly expressed during fibrosis and cancer development, and are potential diagnostic and therapeutic targets (4-6).
  1. Zollinger, A.J. and Smith, M.L. (2017) Matrix Biol 60-61, 27-37.
  2. Wang, K. et al. (2016) Cell Mol Bioeng 9, 1-11.
  3. To, W.S. and Midwood, K.S. (2011) Fibrogenesis Tissue Repair 4, 21.
  4. Kaspar, M. et al. (2006) Int J Cancer 118, 1331-9.
  5. Wang, J.P. and Hielscher, A. (2017) J Cancer 8, 674-82.
  6. Zent, J. and Guo, L.W. (2018) Cell Physiol Biochem 49, 848-68.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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