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  3. Firefly Luciferase (E9W9V) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Firefly Luciferase (E9W9V) Rabbit Monoclonal Antibody #25039

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  • WB
  • IF

    Product Specifications

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa) 62
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Firefly Luciferase (E9W9V) Rabbit Monoclonal Antibody recognizes exogenously expressed proteins containing firefly luciferase (P. pyralis). This antibody may also recognize a non-specific band of unknown origin at 70 kDa in rat cell lines.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln230 of firefly luciferase (P. pyralis) protein.

    Background

    Luciferases are a group of enzymes that emit visible light in the presence of oxygen and luciferin. Firefly luciferase (FLuc) is a 62 kDa oxygenase isolated from the firefly, P. pyralis, that produces a yellow-green light with a peak emission wavelength of 550-570 nm (1). FLuc is widely used as a bioluminescent reporter, emitting light through an ATP-dependent reaction involving oxygen, D-luciferin, and a metal cation, typically magnesium (Mg2+) (2). FLuc is often genetically engineered into luciferase-containing plasmids as a reporter that can be measured by detection of firefly luciferase (P. pyralis) protein or its enzymatic activity (2,3).

    Alternate Names

    luciferin 4-monooxygenase

    Database Links

    UniProt ID: P08659


    Entrez-Gene Id: 116160065


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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