FoxK2 (E7C5H) Rabbit mAb #28712
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 79 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Chromatin IP | 1:50 |
Storage
Protocol
Specificity / Sensitivity
FoxK2 (E7C5H) Rabbit mAb recognizes endogenous levels of total FoxK2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala414 of human FoxK2 protein.
Background
Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors defined by the presence of a winged helix DNA-binding domain called a forkhead box (1). In humans, there are over 40 known Fox protein family members, divided into 19 subfamilies, which have evolved to regulate gene transcription in diverse and highly specialized biological contexts throughout development (2). Mutations that disrupt the expression of Fox gene family members have consequently been implicated in a broad array of human disorders, including immunological dysfunction, infertility, speech/language disorders, and cancer (3,4).
FoxK1 and FoxK2 belong to one of the subfamilies of Fox proteins and contain a forkhead-associated (FHA) domain and a conserved forkhead DNA-binding domain (5). FoxK2 was initially identified as a regulator of IL-2 transcription (6). Studies have shown that FoxK2 binds to DNA harboring G/T mismatches and might be involved in DNA mismatch repair (7). FoxK2 also mediates the oncogenic function of adenovirus (Adv) oncoprotein E1A and papillomavirus E6 proteins via association with these proteins (8). Moreover, FoxK2 promotes AP-1-mediated transcriptional regulation by enhancing the recruitment of AP-1 to chromatin (9). FoxK2 is phosphorylated at Ser368 and Ser423 in a CDK1-cyclin B-dependent manner and is involved in cell cycle regulation (10).
- Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
- Jackson, B.C. et al. (2010) Hum Genomics 4, 345-52.
- Hannenhalli, S. and Kaestner, K.H. (2009) Nat Rev Genet 10, 233-40.
- Benayoun, B.A. et al. (2011) Trends Genet 27, 224-32.
- Katoh, M. and Katoh, M. (2004) Int J Mol Med 14, 127-32.
- Li, C. et al. (1991) Proc Natl Acad Sci USA 88, 7739-43.
- Fujii, Y. and Nakamura, M. (2010) J Biochem 147, 705-9.
- Komorek, J. et al. (2010) J Virol 84, 2719-31.
- Ji, Z. et al. (2012) Mol Cell Biol 32, 385-98.
- Marais, A. et al. (2010) J Biol Chem 285, 35728-39.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.