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Fragilis Antibody #9037

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  • WB
  • IP

Inquiry Info. # 9037

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    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 12
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Fragilis Antibody recognizes endogenous levels of total fragilis protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln16 of mouse fragilis protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Fragilis (Ifitm3) belongs to the interferon (IFN)-inducible transmembrane protein family (1), which consists of five highly similar transmembrane proteins that are conserved among mammalian species (2). It is thought to be the first gene to mark the onset of germ cell competence during embryonic development in mice (3). Cells expressing high levels of fragilis protein go on to express stella, a protein found exclusively in lineage-restricted germ cells (3). Studies on fragilis family members could help elucidate the mechanisms through which interferons act as signaling molecules during embryonic development (2).

    Alternate Names

    1110004C05Rik; Cd225; Cdw217; Dispanin subfamily A member 2b; DSPA2b; Fgls; Fragilis; Fragilis protein; ifitm-like protein 1; Ifitm3; IFM3; Interferon induced transmembrane protein 3; Interferon-induced transmembrane protein 3; Interferon-inducible protein 15; IP15; MCG22590; mil; mil-1; Mouse ifitm-like protein 1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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