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48510
Functional Neuron Marker Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit
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Functional Neuron Marker Antibody Sampler Kit #48510

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Functional Neuron Marker Antibody Sampler Kit: Image 1
Western blot analysis of extracts from rat synaptosome, mouse brain, and rat brain tissue using VGLUT1 Antibody.
Functional Neuron Marker Antibody Sampler Kit: Image 2
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human 5-HTR4 (h5-HTR4-Myc/DDK; +), using 5-HTR4 (D8O5K) Rabbit mAb.
Functional Neuron Marker Antibody Sampler Kit: Image 3
Western blot analysis of extracts from human spinal cord, mouse spinal cord, and mouse kidney using ChAT (E4F9G) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Functional Neuron Marker Antibody Sampler Kit: Image 4
Western blot analysis of extracts from mouse and rat tissue using GAD1 (D1F2M) Rabbit mAb (upper) and B-Actin (D6A8) Rabbit mAb #8457 (lower).
Functional Neuron Marker Antibody Sampler Kit: Image 5
Western blot analysis of extracts from neonatal mouse and fetal rat brain using GAD2 (D5G2) XP® Rabbit mAb.
Functional Neuron Marker Antibody Sampler Kit: Image 6
Western blot analysis of extracts from various tissues and cell lines using Tyrosine Hydroxylase (E2L6M) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Functional Neuron Marker Antibody Sampler Kit: Image 7
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Functional Neuron Marker Antibody Sampler Kit: Image 8
Western blot analysis of extracts from mouse brain, mouse lung, rat brain, and human cortex using VGLUT2 (D7D2H) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Functional Neuron Marker Antibody Sampler Kit: Image 9
Western blot analysis of ACHN cells or 293T cells, either mock transfected (-) or transfected (+) with a cDNA expression construct encoding human β2-adrenergic receptor (hβ2AR), using β2-Adrenergic Receptor (D6H2) Rabbit mAb.
Functional Neuron Marker Antibody Sampler Kit: Image 10
Western blot analysis of extracts from NIH/3T3 and PC-12 cells using 5-HTR4 (D8O5K) Rabbit mAb.
Functional Neuron Marker Antibody Sampler Kit: Image 11
Immunoprecipitation of GAD1 protein from mouse brain tissue extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is GAD1 (D1F2M) Rabbit mAb. Western blot analysis was performed using GAD1 (D1F2M) Rabbit mAb.
Functional Neuron Marker Antibody Sampler Kit: Image 12
Confocal immunofluorescent analysis of mouse brain using GAD2 (D5G2) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA marker).
Functional Neuron Marker Antibody Sampler Kit: Image 13
Immunohistochemical analysis of paraffin-embedded mouse brain using Tyrosine Hydroxylase (E2L6M) Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Functional Neuron Marker Antibody Sampler Kit: Image 14
Immunoprecipitation of VGLUT2 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is VGLUT2 (D7D2H) Rabbit mAb. Western blot analysis was performed using VGLUT2 (D7D2H) Rabbit mAb.
Functional Neuron Marker Antibody Sampler Kit: Image 15
Confocal immunofluorescent analysis of rat retina (left) and cerebellum (right) using GAD1 (D1F2M) Rabbit mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Functional Neuron Marker Antibody Sampler Kit: Image 16
Immunohistochemical analysis of paraffin-embedded PC-12 cell pellet (left, positive) or KNRK cell pellet (right, negative) using Tyrosine Hydroxylase (E2L6M) Rabbit mAb
Functional Neuron Marker Antibody Sampler Kit: Image 17
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Tyrosine Hydroxylase (E2L6M) Rabbit mAb.
Functional Neuron Marker Antibody Sampler Kit: Image 18
Immunohistochemical analysis of paraffin-embedded rat small intestine using Tyrosine Hydroxylase (E2L6M) Rabbit mAb.
Functional Neuron Marker Antibody Sampler Kit: Image 19
Immunohistochemical analysis of paraffin-embedded mouse brain using Tyrosine Hydroxylase (E2L6M) Rabbit mAb performed on the Leica BOND Rx.
Functional Neuron Marker Antibody Sampler Kit: Image 20
Immunohistochemical analysis of paraffin-embedded mouse colon using Tyrosine Hydroxylase (E2L6M) Rabbit mAb performed on the Leica BOND Rx.
Functional Neuron Marker Antibody Sampler Kit: Image 21
Confocal immunofluorescent analysis of rat striatum using Tyrosine Hydroxylase (E2L6M) Rabbit mAb (green) and Neurofilament-L (DA2) Mouse mAb #2835 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Functional Neuron Marker Antibody Sampler Kit: Image 22
Confocal immunofluorescent analysis of HCC1419 cells (left, positive) and ACHN cells (right, negative) using Tyrosine Hydroxylase (E2L6M) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue = Hoechst 33342 #4082 (fluorescent DNA dye).
Functional Neuron Marker Antibody Sampler Kit: Image 23
Flow cytometric analysis of KNRK cells (blue, negative) and PC-12 cells (green, positive) using Tyrosine Hydroxylase (E2L6M) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
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To Purchase # 48510
Cat. # Size Qty. Price
48510T		 1 Kit  (8 x 20 microliters)
$ 645

Custom Formulation

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Tyrosine Hydroxylase (E2L6M) Rabbit mAb 58844 20 µl
  • WB
  • IHC
  • IF
  • F
 H M R 55-60 Rabbit IgG
GAD2 (D5G2) XP® Rabbit mAb 5843 20 µl
  • WB
  • IP
  • IF
 H M R 60 Rabbit IgG
VGLUT2 (D7D2H) Rabbit mAb 71555 20 µl
  • WB
  • IP
 H M R 65-70 Rabbit IgG
VGLUT1 Antibody 12331 20 µl
  • WB
 M R 62 Rabbit 
ChAT (E4F9G) Rabbit mAb 27269 20 µl
  • WB
 H M R 71 Rabbit IgG
GAD1 (D1F2M) Rabbit mAb 41318 20 µl
  • WB
  • IP
  • IF
 M R 67 Rabbit IgG
5-HTR4 (D8O5K) Rabbit mAb 13690 20 µl
  • WB
 H M R 40-140 Rabbit IgG
β2-Adrenergic Receptor (D6H2) Rabbit mAb 8513 20 µl
  • WB
 H 50-100 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
 Rab Goat 

Product Description

The Functional Neuron Marker Antibody Sampler Kit provides an economical means of detecting markers that facilitate phenotyping neurons by function. The kit includes enough antibodies to perform at least two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Functional Neuron Marker Antibody Sampler Kit detects endogenous levels of its target protein.

Source / Purification

Monoclonal and polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala15 of human GAD1 protein, Ala361 of human 5-HTR4 protein, the amino terminus of human VGLUT1 and human VGLUT2 protein, and the carboxy terminus of human tyrosine hydroxylase protein, human GAD2 protein, human ChAT protein, and human β2-adrenergic receptor protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines, and acts as a marker for dopaminergic neurons (1,2). Choline O-acetyltransferase (ChAT) is the enzyme that catalyzes synthesis of acetylcholine (ACh) in the central and peripheral nervous system. ChAT is found in high levels within cholinergic neurons and can be used to measure their functional states (3). Vesicular glutamate transporters 1 and 2 (VGLUT1 and VGLUT2) are responsible for transporting the excitatory neurotransmitter glutamate into synaptic vesicles of glutamatergic neurons. VGLUT1 and VGLUT2 are complimentarily expressed and act as markers for glutamatergic neurons (4). Glutamate decarboxylase (GAD) is the main enzyme that synthesizes GABA from glutamate. GABA producing neurons, called GABAergic neurons, utilize GABA as their major inhibitory neurotransmitter with both isoforms of GAD, GAD1, and GAD2, acting as functional markers for these neurons (5). β2-adrenergic receptor (β2AR) is a G protein-coupled receptor (GPCR) that mediates the actions of catecholamines, mainly through stimulation by epinephrine (adrenaline), in the central and peripheral nervous system (6,7). Serotonin receptor 4 (5-HTR4) is an excitatory GPCR that activates the cyclic AMP (cAMP)-PKA pathway (8,9). 5-HTR4 is located post-synaptically on serotonergic neurons (10).
  1. Kumer, S.C. and Vrana, K.E. (1996) J Neurochem 67, 443-62.
  2. Weihe, E. et al. Cell Mol Neurobiol 26, 659-78.
  3. Oda, Y. (1999) Pathol Int 49, 921-37.
  4. Ziegler, D.R. et al. (2002) J Comp Neurol 448, 217-29.
  5. Le, T.N. et al. (2017) J Neurosci 37, 8816-8829.
  6. Wu, Y. et al. (2021) Biomolecules 11, 936. doi: 10.3390/biom11070936.
  7. Wallukat, G. (2002) Herz 27, 683-90.
  8. Nichols, D.E. and Nichols, C.D. (2008) Chem Rev 108, 1614-41.
  9. Karayol, R. et al. (2021) Mol Psychiatry 26, 2334-2349.
  10. Samuels, B.A. et al. (2016) Neuroscientist 22, 26-45.

Pathways

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Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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