Render Target: STATIC
Render Timestamp: 2024-10-11T09:39:39.925Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-17 22:05:21.302
Product last modified at: 2024-09-19T08:00:15.596Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Gab3 (F3W6T) Rabbit mAb #34612

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 70, 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Gab3 (F3W6T) Rabbit mAb recognizes endogenous levels of total Gab3 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu481 of human Gab3 protein.

    Background

    Grb2-associated-binding protein 3 (Gab3) is a member of the Grb-associated binder (Gab) family of adaptor proteins that support the assembly of activated signaling complexes through docking and scaffolding functions (1). The Gab family proteins, Gab1-3, contain a conserved N-terminal pleckstrin homology (PH) domain and multiple phosphorylation sites specific for tyrosine kinases (2). The PH-domain interacts with lipids like phosphatidylinositol-3,4,5-triphosphate (PIP3) to mediate membrane recruitment, and the phosphorylated tyrosines act as recruitment sites for SH2 domain-containing proteins (3,4). Gab3 is expressed in hematopoietic lineage cells, including natural killer (NK) cells, mast cells, and memory CD8+ T cells (5). Gab3 plays an important role in peripheral NK cell expansion and is essential for IL-2/IL-15 receptor-mediated MAPK signaling in NK cells (6). Gab3 expression is associated with shorter progression-free survival in ovarian cancer and may be a promising biomarker to predict immunotherapeutic responses in lung adenocarcinoma (LUAD) and melanoma (7-9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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