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GADD34 (E3S6N) Rabbit Monoclonal Antibody

GADD34 (E3S6N) Rabbit Monoclonal Antibody #41222

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Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	110
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

GADD34 (E3S6N) Rabbit Monoclonal Antibody recognizes endogenous levels of total GADD34 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala69 of human GADD34 protein.

Background

The growth arrest and DNA damage-inducible protein, GADD34, is essential for cell stress responses. It is induced by stresses such as DNA damage and endoplasmic reticulum (ER) stress. ER stress stimulates the unfolded protein response (UPR), increasing phosphorylation of Ser51 in the α subunit of eukaryotic translation initiation factor 2 (eIF2α). This change represses translation. Phosphorylation of eIF2α also increases GADD34 expression. GADD34 binds to type 1 protein phosphatase 1 (PP1), a serine/threonine phosphatase, and promotes PP1 to dephosphorylate eIF2α (1,2). GADD34 expression is correlated with eIF2α dephosphorylation late in the stress response, suggesting a critical role of GADD34 in a negative feedback loop to reduce stress response signaling and shift cells from protein synthesis inhibition to stress-induced gene expression (3).

Alternate Names

GADD34; Growth arrest and DNA damage-inducible protein GADD34; growth arrest and DNA-damage-inducible 34; Myeloid differentiation primary response protein MyD116 homolog; PPP1R15A; PR15A; Protein phosphatase 1 regulatory subunit 15A; protein phosphatase 1, regulatory (inhibitor) subunit 15A

Database Links

UniProt ID: O75807

Entrez-Gene Id: 23645

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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