Render Target: STATIC
Render Timestamp: 2024-11-07T10:53:50.024Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:58:18.038
Product last modified at: 2024-11-01T14:15:10.142Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GADD34 (E3S6N) Rabbit mAb #41222

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 110
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GADD34 (E3S6N) Rabbit mAb recognizes endogenous levels of total GADD34 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala69 of human GADD34 protein.

    Background

    The growth arrest and DNA damage-inducible protein, GADD34, is essential for cell stress responses. It is induced by stresses such as DNA damage and endoplasmic reticulum (ER) stress. ER stress stimulates the unfolded protein response (UPR), increasing phosphorylation of Ser51 in the α subunit of eukaryotic translation initiation factor 2 (eIF2α). This change represses translation. Phosphorylation of eIF2α also increases GADD34 expression. GADD34 binds to type 1 protein phosphatase 1 (PP1), a serine/threonine phosphatase, and promotes PP1 to dephosphorylate eIF2α (1,2). GADD34 expression is correlated with eIF2α dephosphorylation late in the stress response, suggesting a critical role of GADD34 in a negative feedback loop to reduce stress response signaling and shift cells from protein synthesis inhibition to stress-induced gene expression (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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