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70310
Galectin-3/LGALS3 (D4I2R) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

Galectin-3/LGALS3 (D4I2R) XP® Rabbit mAb (BSA and Azide Free) #70310

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: Galectin-3/LGALS3 (D4I2R) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using Galectin-3/LGALS3 (D4I2R) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Galectin-3/LGALS3 (D4I2R) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Galectin-3/LGALS3 (D4I2R) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Galectin-3/LGALS3 (D4I2R) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded SK-MEL-28 (left) and LNCaP (right) cell pellets using Galectin-3/LGALS3 (D4I2R) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Galectin-3/LGALS3 (D4I2R) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Galectin-3/LGALS3 (D4I2R) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: Galectin-3/LGALS3 (D4I2R) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of SK-MEL-28 (left) and LNCaP (right) cells, using Galectin-3/LGALS3 (D4I2R) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Data were generated using the standard formulation of this product.
To Purchase # 70310
Cat. # Size Qty. Price
70310SF
100 µg
$ 758

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 28
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #87985. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #87985

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Galectin-3/LGALS3 (D4I2R) XP® Rabbit mAbrecognizes endogenous levels of total Galectin-3/LGALS3 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Galectin-3/LGALS3 protein.

Background

Galectins are a family of β-galactose binding proteins that are characterized by an affinity for poly-N-acetyllactosamine-enriched glycoconjugates and a carbohydrate-binding site (1,2). Members of the galectin family have been implicated in a variety of biological functions, including cell adhesion (3), growth regulation (4), cytokine production (5), T cell apoptosis (6), and immune responses (7).

Galectin-3/LGALS3 is involved in several diverse biological functions. Galectin-3/LGALS3 binds IgE (8). Galectin-3/LGALS3 is an unusual protein in that it can be found both extracellularly and intracellularly. Intracellularly, Galectin-3/LGALS3 can localize to the cytoplasm, nucleus, or both, depending on cell type and experimental conditions. Nuclear Galectin-3/LGALS3 has been identified as a pre-mRNA splicing factor (9). Galectin-3/LGALS3 production has been shown to increase during inflammation and in obesity, and the protein itself can have an inflammatory effect under certain conditions (10). Galectin-3/LGALS3 forms a complex with α3, β1 integrin to act as a surface receptor on endothelial cells for the NG2 proteoglycan, which triggers cell motility and angiogenesis (11). In addition to these functions, Galectin-3/LGALS3 is also a required factor for the terminal differentiation of epithelial cells (12).
  1. Barondes, S.H. et al. (1994) Cell 76, 597-8.
  2. Barondes, S.H. et al. (1994) J Biol Chem 269, 20807-10.
  3. Offner, H. et al. (1990) J Neuroimmunol 28, 177-84.
  4. Wells, V. and Mallucci, L. (1991) Cell 64, 91-7.
  5. Filer, A. et al. (2009) Arthritis Rheum 60, 1604-14.
  6. Perillo, N.L. et al. (1995) Nature 378, 736-9.
  7. Cooper, D.N. et al. (1991) J Cell Biol 115, 1437-48.
  8. Platzer, B. et al. (2011) Immunol Lett 141, 36-44.
  9. Haudek, K.C. et al. (2010) Biochim Biophys Acta 1800, 181-9.
  10. Pang, J. et al. (2013) PLoS One 8, e57915.
  11. Fukushi, J. et al. (2004) Mol Biol Cell 15, 3580-90.
  12. Hikita, C. et al. (2000) J Cell Biol 151, 1235-46.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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