Cell Signaling Technology Logo
1% for the planet logo
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GAMT (F1A7Z) Rabbit mAb #69029

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 26
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GAMT (F1A7Z) Rabbit mAb recognizes endogenous levels of total GAMT protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp130 of human GAMT protein.

    Background

    Guanidinoacetate N-methyltransferase (GAMT) is an enzyme member of the transferase protein family that functions in creatine synthesis. Creatine is a naturally occurring compound that plays a role in maintaining ATP levels within metabolically active tissues such as muscle and brain. While creatine is mainly synthesized in the kidneys and liver, it is also endogenously synthesized in the brain (1,2). Creatine synthesis occurs through the conversion of arginine and glycine to guanidinoacetate (GAA) by arginine:glycine amidinotransferase (AGAT). GAA is then methylated by GAMT, forming creatine (3). In the brain, this system is essential to maintaining the development and function of the central nervous system, with deficiency in either GAMT or AGAT leading to neurological consequences. GAMT deficiency is a disorder caused by mutations in the GAMT gene, which disrupts the creatine synthesis pathway, leading to developmental delays, varying intellectual disability, and seizure disorders (4,5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.