GAP43 (E6L2W) Mouse mAb #41653
- WB
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 38, 43 |
| Source/Isotype | Mouse IgG2b |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
Protocol
Specificity / Sensitivity
GAP43 (E6L2W) Mouse mAb recognizes endogenous levels of total GAP43 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human GAP43 protein.
Background
GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity (1). Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca2+ and affects the ability of GAP43 to bind calmodulin (2,3). GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex (4,5). Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer's Disease (6,7).
- Biewenga, J.E. et al. (1996) Acta Biochim Pol 43, 327-38.
- Dekker, L.V. and Parker, P.J. (1997) J Biol Chem 272, 12747-53.
- Chapman, E.R. et al. (1991) J Biol Chem 266, 207-13.
- Aigner, L. and Caroni, P. (1993) J Cell Biol 123, 417-29.
- Shen, Y. et al. (2008) Cerebellum 7, 451-66.
- Sower, A.C. et al. (1995) Mol Chem Neuropathol 24, 1-11.
- de la Monte, S.M. et al. (1995) Am J Pathol 147, 934-46.
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