Gasdermin A Antibody #49307
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 49 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Gasdermin A Antibody recognizes endogenous levels of total Gasdermin A protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly256 of human Gasdermin A protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The gasdermin family, which includes GSDMA, GSDMB, GSDMC, GSDMD, and GSDME, has been shown to play a role in inflammation and cell death. Gasdermin D has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7).
Gasdermin A (GSDMA) is preferentially expressed in the epithelium of the skin and gastrointestinal tract and is frequently suppressed in gastric cancer (8-10). Mice express three Gasdermin A genes termed GSDMA1-3 (8). The role of Gasdermin A has been associated with cellular differentiation, apoptosis, pyroptosis, and autophagy (10-15). Expression of an N-terminal fragment of GSDMA3, but not the full-length protein, induces pyroptosis, but the mechanisms of any cleavage of GSDMA3 are unknown (12). The most widely studied mouse form, GSDMA3, is expressed in mouse keratinocytes and is associated with skin differentiation and inflammation, and a dominant mutation has been found to play a causative role in alopecia (16,17).
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- Shi, J. et al. (2015) Nature 526, 660-5.
- Broz, P. and Dixit, V.M. (2016) Nat Rev Immunol 16, 407-20.
- Aglietti, R.A. et al. (2016) Proc Natl Acad Sci USA 113, 7858-63.
- Ding, J. et al. (2016) Nature 535, 111-6.
- Liu, X. et al. (2016) Nature 535, 153-8.
- Sborgi, L. et al. (2016) EMBO J 35, 1766-78.
- Tamura, M. et al. (2007) Genomics 89, 618-29.
- Saeki, N. et al. (2000) Mamm Genome 11, 718-24.
- Saeki, N. et al. (2007) Oncogene 26, 6488-98.
- Li, J. et al. (2010) Biochem Biophys Res Commun 403, 18-23.
- Shi, J. et al. (2015) Nature 526, 660-5.
- Shi, P. et al. (2015) Biochem J 468, 325-36.
- Lei, M. et al. (2011) Histochem Cell Biol 136, 335-43.
- Lei, M. et al. (2012) Histochem Cell Biol 138, 385-96.
- Runkel, F. et al. (2004) Genomics 84, 824-35.
- Zhou, Y. et al. (2012) Am J Pathol 180, 763-74.
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