|93709||Gasdermin D (L60) Antibody||
||H M R|
|MW (kDa)||29, 53|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Gasdermin D Antibody recognizes endogenous levels of total Gasdermin D protein. This antibody detects the N-terminal fragment of Gasdermin D upon proteolytic cleavage.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg140 of human Gasdermin D protein. Antibodies are purified by protein A and peptide affinity chromatography.
Gasdermin D (GSDMD), a member of the gasdermin family that includes GSDMA, GSDMB, and GSMDC, has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSMDM-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7).
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KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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