GATA-1 (D52H6) XP® Rabbit mAb (BSA and Azide Free) #40977
- WB
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 43 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
GATA-1 (D52H6) XP® Rabbit mAb (BSA and Azide Free) detects endogenous levels of GATA-1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu13 of human GATA-1.
Background
GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA-binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3).
GATA-1 is the founding member of the GATA family and is required for erythroid and megakaryocytic cell development (4,5). Mutations in the corresponding GATA-1 gene are linked to many human diseases, including acute megakaryoblastic leukemia in Down Syndrome children (DS-AMKL), X-linked thrombocytopenia, and gray platelet syndrome (6-9).
- Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
- Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
- Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
- Pevny, L. et al. (1991) Nature 349, 257-60.
- Fujiwara, Y. et al. (1996) Proc Natl Acad Sci USA 93, 12355-8.
- Wechsler, J. et al. (2002) Nat Genet 32, 148-52.
- Cantor, A.B. (2005) Int J Hematol 81, 378-84.
- Mehaffey, M.G. et al. (2001) Blood 98, 2681-8.
- Tubman, V.N. et al. (2007) Blood 109, 3297-9.
Limited Uses
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