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10630
GATA-3 (D13C9) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

GATA-3 (D13C9) XP® Rabbit mAb (BSA and Azide Free) #10630

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  1. WB
  2. IHC
  3. IF
  4. F
Western Blotting Image 1: GATA-3 (D13C9) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from CEM and SH-SY5Y cells using GATA-3 (D13C9) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: GATA-3 (D13C9) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GATA-3 (D13C9) XP® Rabbit mAb (left) or Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: GATA-3 (D13C9) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded cell pellets, MCF7 (left) or HUVEC (right), using GATA-3 (D13C9) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: GATA-3 (D13C9) XP® Rabbit mAb (BSA and Azide Free)
Confocal Immunofluorescent analysis of MCF7 cells (left) and HUVEC (right) using GATA-3 (D13C9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Data were generated using the standard formulation of this product.
Flow Cytometry Image 1: GATA-3 (D13C9) XP® Rabbit mAb (BSA and Azide Free)
Flow cytometric analysis of THP-1 cells (blue) and MCF7 cells (green) using GATA-3 (D13C9) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 10630
Cat. # Size Qty. Price
10630SF
100 µg
$ 746

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 48
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier-free version of product #5852. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #5852.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

GATA-3 (D13C9) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total GATA-3 protein.

Species Reactivity:

Human, Mouse

Species predicted to react based on 100% sequence homology:

Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GATA-3 protein.

Background

GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA-binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3).

GATA-3 is a critical regulator of development of various systems in both mouse and human (4). GATA-3 mouse embryos die between E11 and E12 due to growth retardation and deformities in the brain and spinal cord (5). The function of GATA-3 has been extensively studied in T cell development and has recently been shown to be a downstream target of Notch in Notch-mediated differentiation of TH2 cells (6,7). It is expressed in both hematopoietic and non-hematopoietic tissues, including the kidney, skin, mammary gland, and central nervous system (8-10). Decreased expression of GATA-3 in luminal breast cancer is associated with poor clinical outcome. GATA-3 expression level may therefore be a promising prognostic biomarker (11). Haploinsufficiency of GATA-3 results in Barakat syndrome in humans, a condition characterized by sensorineural deafness and renal dysplasia (12).
  1. Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
  2. Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
  3. Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
  4. Debacker, C. et al. (1999) Mech Dev 85, 183-7.
  5. Pandolfi, P.P. et al. (1995) Nat Genet 11, 40-4.
  6. Ho, I.C. et al. (2009) Nat Rev Immunol 9, 125-35.
  7. Amsen, D. et al. (2007) Immunity 27, 89-99.
  8. Grote, D. et al. (2008) PLoS Genet 4, e1000316.
  9. Kaufman, C.K. et al. (2003) Genes Dev 17, 2108-22.
  10. Kouros-Mehr, H. et al. (2006) Cell 127, 1041-55.
  11. Chou, J. et al. (2010) J Cell Physiol 222, 42-9.
  12. Van Esch, H. et al. (2000) Nature 406, 419-22.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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