GATAD2A (E7B5B) Rabbit mAb #17705
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70, 73 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:50 |
Storage
Protocol
Specificity / Sensitivity
GATAD2A (E7B5B) Rabbit mAb recognizes endogenous levels of total GATAD2A protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro627 of human GATAD2A protein.
Background
GATAD2A and GATAD2B, also known as p66 alpha and p66 beta, are related members of the nucleosome remodeling and deacetylase (NuRD) complex (1). GATAD2A interacts with MBD2 to recruit the deacetylase core of the NuRD complex to repress target genes, a process that is enhanced by the sumoylation of GATAD2A at Lys30 and Lys487 (2-5). In addition to being sumoylated, GATAD2A has also been shown to be phosphorylated by AMPK in the memory formation process (6). Depletion of GATAD2A in order to reduce NuRD-dependent repression of key pluripotency genes is being explored in the context of induced pluripotent stem cell (iPSC) generation (7,8).
- Brackertz, M. et al. (2002) J Biol Chem 277, 40958-66.
- Brackertz, M. et al. (2006) Nucleic Acids Res 34, 397-406.
- Gnanapragasam, M.N. et al. (2011) Proc Natl Acad Sci U S A 108, 7487-92.
- Desai, M.A. et al. (2015) Nucleic Acids Res 43, 3100-13.
- Gong, Z. et al. (2006) Mol Cell Biol 26, 4519-28.
- Ding, X. et al. (2017) Nat Neurosci 20, 690-9.
- Mor, N. et al. (2018) Cell Stem Cell 23, 412-425.e10.
- Zviran, A. et al. (2019) Cell Stem Cell 24, 328-341.e9.
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