|REACTIVITY||H M R Mk|
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Human, Mouse, Rat, Monkey
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly521 of human GATAD2B protein.
GATAD2A and GATAD2B, also known as p66 alpha and p66 beta, are related members of the nucleosome remodeling and deacetylase (NuRD) complex (1). GATAD2B interacts with MBD2 to recruit the deacetylase core of the NuRD complex to repress target genes, a process that is enhanced by the sumoylation of GATAD2B at Lys33 (2-5). In addition to being sumoylated, GATAD2B has also been shown to be phosphorylated by Mkk6 during reprogramming (6). The GATAD2B protein contains an aggregation-prone region of its C-terminus which interacts with other NuRD members CHD4 and CDK2AP1 (7). Deletion of GATAD2B has been shown to be associated with severe intellectual disability (8,9).
UniProt ID: Q8WXI9
Entrez-Gene Id: 57459
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