GATAD2B (E2G4R) Rabbit mAb #52626
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 77 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
GATAD2B (E2G4R) Rabbit mAb recognizes endogenous levels of total GATAD2B protein. This antibody does not cross-react with GATAD2A protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly521 of human GATAD2B protein.
Background
GATAD2A and GATAD2B, also known as p66 alpha and p66 beta, are related members of the nucleosome remodeling and deacetylase (NuRD) complex (1). GATAD2B interacts with MBD2 to recruit the deacetylase core of the NuRD complex to repress target genes, a process that is enhanced by the sumoylation of GATAD2B at Lys33 (2-5). In addition to being sumoylated, GATAD2B has also been shown to be phosphorylated by Mkk6 during reprogramming (6). The GATAD2B protein contains an aggregation-prone region of its C-terminus which interacts with other NuRD members CHD4 and CDK2AP1 (7). Deletion of GATAD2B has been shown to be associated with severe intellectual disability (8,9).
- Brackertz, M. et al. (2002) J Biol Chem 277, 40958-66.
- Brackertz, M. et al. (2006) Nucleic Acids Res 34, 397-406.
- Gnanapragasam, M.N. et al. (2011) Proc Natl Acad Sci U S A 108, 7487-92.
- Desai, M.A. et al. (2015) Nucleic Acids Res 43, 3100-13.
- Gong, Z. et al. (2006) Mol Cell Biol 26, 4519-28.
- Xing, G. et al. (2021) Cell Death Differ, doi: 10.1038/s41418-021-00923-8.
- Sharifi Tabar, M. et al. (2022) FEBS J 289, 199-214.
- Willemsen, M.H. et al. (2013) J Med Genet 50, 507-14.
- Luo, X. et al. (2017) J Hum Genet 62, 513-516.
Limited Uses
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