Render Target: STATIC
Render Timestamp: 2024-12-13T12:18:24.740Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:57:52.461
Product last modified at: 2024-12-06T17:30:08.967Z
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PDP - Template Name: Monoclonal Antibody (Epitope Tag)
PDP - Template ID: *******f943b5c

GFP (5G4) Mouse mAb #55494

Filter:
  • IP
  • IF

    Supporting Data

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Mouse IgG2b
    Application Key:
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GFP (5G4) Mouse mAb detects native GFP and YFP-tagged proteins exogenously expressed in cells, but does not detect denatured GFP. This antibody does not detect RFP-tagged proteins. Please note that the GFP and YFP tags add approximately 27 kDa to the molecular weight of the fusion protein.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with native GFP.

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    The green fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage, and defines subcellular protein localization (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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