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  2. Primary Antibodies /
  3. GHF-1 (F9C3D) Rabbit mAb
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GHF-1 (F9C3D) Rabbit mAb #50830

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  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 29
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    MW (kDa) 29

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GHF-1 (F9C3D) Rabbit mAb recognizes endogenous levels of total GHF-1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu86 of human GHF-1 protein.

    Background

    Growth hormone factor 1 (GHF-1), also known as Pit-1, is a POU domain transcription factor essential for the development and function of specific anterior pituitary cells (i.e., somatotrophs, lactotrophs, and thyrotrophs) (2). It activates the genes for growth hormone, prolactin, and TSH beta, crucial for hormone production and pituitary cell lineage determination (1,2). Mutations in POU1F1 (the gene encoding GHF-1) are a major cause of combined pituitary hormone deficiency and isolated growth hormone deficiency, leading to impaired hormone secretion and developmental issues (3). While GHF-1 is crucial for normal pituitary development, its role in pituitary tumorigenesis is complex and not fully elucidated. Some pituitary adenomas, particularly those of the somatotroph and lactotroph lineages, express GHF-1. However, POU1F1 mutations are not a common cause of sporadic pituitary adenomas. Aberrant GHF-1 expression or activity might contribute to tumor progression in some cases, but further research is needed (4,5).

    Database Links

    UniProt ID: P28069


    Entrez-Gene Id: 5449


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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