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GLIPR2 (F7H7V) Rabbit mAb #86955

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  • WB
  • IHC

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 17
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GLIPR2 (F7H7V) Rabbit mAb recognizes endogenous levels of total GLIPR2 protein. An unknown background band is detected at 65 kDa in some human and mouse cell lines.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro135 of human GLIPR2 protein.

    Background

    Glioma pathogenesis-related protein 2 (GLIPR2), also known as Golgi-associated PR-1 protein (GAPR-1), is a Golgi-associated protein belonging to the PR-1 protein family (1). GLIPR2 contains a sperm-coating protein (SCP) domain, also known as a CAP domain; however, in contrast to other SCP domain-containing proteins, it does not have a signal peptide and is not secreted via the classical secretory pathway (2). GLIPR2 is expressed in monocytes, macrophages, natural killer (NK) cells, and T cell subsets (3). GLIPR2 binds Beclin-1 and is a negative regulator of autophagy that inhibits PtdIns3K-C1 complex activity (4,5). GLIPR2 promotes epithelial to mesenchymal transition in vitro, and is expressed at high levels in fibrotic kidney (6). GLIPR2 has been identified as a marker for type 1 migratory dendritic cells (DC1s) (7) and a potential biomarker and tumor suppressor in a pan-cancer analysis (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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