Render Target: STATIC
Render Timestamp: 2024-12-02T11:19:47.007Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-30 01:59:39.022
Product last modified at: 2024-10-22T20:00:14.347Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GLRX1 (E4D6J) Rabbit mAb #92350

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 10
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GLRX1 (E4D6J) Rabbit mAb recognizes endogenous levels of total GLRX1 protein. This antibody does not cross-react with GLRX2 protein. This antibody detects a nonspecific band at 42 kDa.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GLRX1 protein.

    Background

    Glutaredoxin-1 (GLRX1, GRX1), also known as thioltransferase-1 (TTase-1), is a thiol disulfide oxidoreductase that catalyzes protein deglutathionylation using glutathione as a cofactor. This small enzyme has a key role in redox homeostasis in the cell (1,2). Studies of mouse models show that GLRX1 is critical in maintaining hepatic lipid homeostasis and preventing fatty liver disease (3). In addition, fibrotic livers have reduced levels of GLRX1 and enhanced levels of protein S-glutathionylation. Furthermore, overexpression of GLRX1 suppresses protein S-glutathionylation and liver fibrosis (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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