GLRX1 (E4D6J) Rabbit mAb #92350
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 10 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Protocol
Specificity / Sensitivity
GLRX1 (E4D6J) Rabbit mAb recognizes endogenous levels of total GLRX1 protein. This antibody does not cross-react with GLRX2 protein. This antibody detects a nonspecific band at 42 kDa.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GLRX1 protein.
Background
Glutaredoxin-1 (GLRX1, GRX1), also known as thioltransferase-1 (TTase-1), is a thiol disulfide oxidoreductase that catalyzes protein deglutathionylation using glutathione as a cofactor. This small enzyme has a key role in redox homeostasis in the cell (1,2). Studies of mouse models show that GLRX1 is critical in maintaining hepatic lipid homeostasis and preventing fatty liver disease (3). In addition, fibrotic livers have reduced levels of GLRX1 and enhanced levels of protein S-glutathionylation. Furthermore, overexpression of GLRX1 suppresses protein S-glutathionylation and liver fibrosis (4).
Limited Uses
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