Immunohistochemical analysis of paraffin-embedded human colon carcinoma using GLUL (D2O3F) Rabbit mAb (IHC Formulated).
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using GLUL (D2O3F) Rabbit mAb (IHC Formulated).
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using GLUL (D2O3F) Rabbit mAb (IHC Formulated).
Immunohistochemical analysis of paraffin-embedded HeLa cell pellet (left, positive) or U-138 MG cell pellet (right, negative) using GLUL (D2O3F) Rabbit mAb (IHC Formulated).
Immunohistochemical analysis of paraffin-embedded rat liver using GLUL (D2O3F) Rabbit mAb (IHC Formulated).
|REACTIVITY||H M R|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
|DETECTION REAGENT/SUBSTRATE COMPATIBILITY|
|SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114||SignalStain® Boost IHC Detection Reagent (AP, Rabbit) #18653|
|SignalStain® DAB Substrate Kit #8059||SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713|
|SignalStain® Vivid Purple Peroxidase Substrate Kit #96632|
NOTE: Use of detection reagents other than those specified in this protocol may require further optimization of the primary antibody to account for the different sensitivities of the detection reagents.
posted February 2010
revised April 2020
Protocol Id: 283
GLUL (D2O3F) Rabbit mAb (IHC Formulated) recognizes endogenous levels of total GLUL protein.Species Reactivity:
Human, Mouse, Rat
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala297 of human GLUL protein.
Glutamate-ammonia ligase (GLUL), also known as glutamine synthetase (GS), catalyzes the de novo synthesis of glutamine from glutamate and ammonia. GLUL is ubiquitously expressed with particularly high expression in the muscle, liver, and brain (1). GLUL expression is elevated in various cancers. Its expression is upregulated by oncogenic c-Myc (2). High expression of GLUL in breast cancer patients is associated with larger tumor size and high level of HER2 expression. It is a predictor of poor survival in patients with glioma and liver cancers (3-6).
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SignalStain is a trademark of Cell Signaling Technology, Inc.