Immunohistochemical analysis of paraffin-embedded Hep G2 cell pellet (left, high-expressing) or MOLT-4 cell pellet (right, low-expressing) using Glut1 (IHC404) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Glut1 (IHC404) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human oral squamous cell carcinoma using Glut1 (IHC404) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the tonsil using Glut1 (IHC404) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using Glut1 (IHC404) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human neuroendocrine lung carcinoma using Glut1 (IHC404) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded normal human lung using Glut1 (IHC404) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using Glut1 (IHC404) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Glut1 (IHC404) Mouse mAb (left) compared to concentration-matched Mouse (E5Y6Q) mAb IgG2a Isotype Control #61656 (right).
Immunohistochemical analysis of paraffin-embedded normal human testis using Glut1 (IHC404) Mouse mAb.
|Immunohistochemistry (Paraffin)||1:1600 - 1:3200|
Supplied in a Tris-based buffer with 1% BSA and less than 0.1% sodium azide. Stable for 24 months when stored at 4°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
|SignalStain® Boost IHC Detection Reagent (HRP, Mouse) #8125||SignalStain® Boost IHC Detection Reagent (AP, Mouse) #31926|
|SignalStain® DAB Substrate Kit #8059||SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713|
|SignalStain® Vivid Purple Peroxidase Substrate Kit #96632|
NOTE: Use of detection reagents other than those specified in this protocol may require further optimization of the primary antibody to account for the different sensitivities of the detection reagents.
posted February 2010
revised June 2020
Protocol Id: 280
Glut1 (IHC404) Mouse mAb recognizes endogenous levels of total Glut1 protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the C terminal of Glut1 protein.
Glucose transporter 1 (Glut1, SLC2A1) is a widely expressed transport protein that displays a broad range of substrate specificity in transporting a number of different aldose sugars as well as an oxidized form of vitamin C into cells (1,2). Glut1 is responsible for the basal-level uptake of glucose from the blood through facilitated diffusion (2). Research studies show that Glut1 and the transcription factor HIF-1α mediate the regulation of glycolysis by O-GlcNAcylation in cancer cells (3). Additional studies demonstrate that Glut1 is required for CD4 T cell activation and is critical for the expansion and survival of T effector (Teff) cells (4). Mutations in the corresponding SLC2A1 gene cause GLUT1 deficiency syndromes (GLUT1DS1, GLUT1DS2), a pair of neurologic disorders characterized by delayed development, seizures, spasticity, paroxysmal exercise-induced dyskinesia, and acquired microcephaly (5,6). Two other neurologic disorders - dystonia-9 (DYT9) and susceptibility to idiopathic generalized epilepsy 12 (EIG12) - are also caused by mutations in the SLC2A1 gene (7,8).
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