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2299
Glut4 Antibody
Primary Antibodies
Polyclonal Antibody

Glut4 Antibody #2299

Reviews ()
Citations (14)

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# Product Name Application Reactivity
  • WB
H M R

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 45
SOURCE Rabbit

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Glut4 Antibody detects endogenous levels of total Glut4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence of human Glut4 (around Q6). Antibodies are purified by protein A and peptide affinity chromatography.

Background

A group of related glucose transporters (Glut1-5 and 7) mediate the facilitated diffusion of glucose in nonepithelial mammalian tissues. Within insulin-responsive tissues such as muscle and fat, Glut1 contributes to basal glucose uptake while Glut4 is responsible for insulin-stimulated glucose transport (1-3). Glut4 is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin stimulation (1,2,4). Translocation of Glut4 is dependent on Akt, which may act by phosphorylating AS160, a RabGAP protein involved in membrane trafficking (5).

  1. Marette, A. et al. (1992) Am J Physiol 263, C443-52.
  2. Slot, J.W. et al. (1991) Proc Natl Acad Sci U S A 88, 7815-9.
  3. James, D.E. et al. (1989) Nature 338, 83-7.
  4. Barrett, M.P. et al. (1999) Curr Opin Cell Biol 11, 496-502.
  5. Zeigerer, A. et al. (2004) Mol Biol Cell 15, 4406-15.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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