Render Target: STATIC
Render Timestamp: 2024-12-13T11:39:52.814Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:59:29.693
Product last modified at: 2024-11-11T13:30:09.709Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GNAS/Gαs (E4G1G) Rabbit mAb #47342

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 42, 45
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GNAS/Gαs (E4G1G) Rabbit mAb recognizes endogenous levels of total GNAS/Gαs protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GNAS/Gαs protein.

    Background

    G protein-coupled receptors (GPCRs) transmit extracellular signals through heterotrimeric G proteins (guanine nucleotide-binding proteins) (1). G proteins have three main subunits: Gα, Gβ, and Gγ. In the quiescent state, the GDP bound Gα is associated with Gβγ. Binding of ligands to GPCR causes the exchange of GDP for GTP on Gα, leading to its dissociation from the Gβγ complex (2). The released GTP-Gα further activates downstream effectors to initiate signaling events. G-protein-mediated signaling is terminated by hydrolysis of GTP by Gα intrinsic GTP hydrolase and reassociation of Gα with Gβγ to form the inactive heterotrimer.

    There are four subclasses of Gα protein, Gαs, Gαi/o, Gαq/11, and Gα12/13 (3), and each is linked to a unique signaling pathway. Gαs is encoded by the GNAS gene. This G protein subunit is associated with a wide spectrum of disorders (4). The GTP bound Gαs activates adenylyl cyclase, which catalyzes the conversion of ATP into the second messenger cAMP and initiates the downstream PKA pathway (5). Mutations on residue Arg201 or Gln227 result in loss of GTP hydrolase activity (6), leading to constitutive activation of Gαs, which contributes to tumor progression (7,8), fibrous dysplasia of bone (9), and McCune-Albright syndrome, a disorder that causes abnormal skin pigmentation, scar tissue formation on bone, and growth-regulating gland malfunction (10). Heterozygous loss-of-function mutations in Gαs lead to Albright hereditary osteodystrophy (AHO), a disease characterized by short stature, obesity, brachydactyly, subcutaneous ossification, and dental deficits (11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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