GNLY (E1V5M) Rabbit mAb #59286
- WB
- IP
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 15 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:3200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #91378.
Protocol
Specificity / Sensitivity
GNLY (E1V5M) Rabbit mAb recognizes endogenous levels of total GNLY protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GNLY protein.
Background
Granulysin (GNLY) was originally identified as a late activation marker in T cells, and it is expressed by killer lymphocytes in most mammals, but not rodents. GNLY is largely confined to cytotoxic granules but can be secreted by killer cells, especially those expressing high levels of GNLY, such as decidual natural killer cells (1-3). GNLY is produced as a 15 kDa protein and is processed into a 9 kDa active pore-forming fragment by proteolytic removal of peptides from both the N- and C-termini. Moreover, the 15 kDa form was reported to function as an immune alarmin, causing the maturation and migration of antigen-presenting cells and other cells of the immune system (4-7). Unlike perforin, a cholesterol-dependent pore-forming protein that preferentially permeabilizes mammalian membranes, GNLY is inhibited by cholesterol and forms pores much more efficiently in microbial than mammalian membranes, and it plays an important role against bacteria, fungi, and parasite infections (8,9).
- Jongstra, J. et al. (1987) J Exp Med 165, 601-14.
- Pitabut, N. et al. (2011) Microbiol Immunol 55, 565-73.
- Crespo, Â.C. et al. (2020) Cell 182, 1125-1139.e18.
- Hanson, D.A. et al. (1999) Mol Immunol 36, 413-22.
- Tewary, P. et al. (2010) Blood 116, 3465-74.
- Clayberger, C. et al. (2012) J Immunol 188, 6119-26.
- Sparrow, E. and Bodman-Smith, M.D. (2020) Immunol Lett 217, 126-132.
- Kumar, J. et al. (2001) Expert Opin Investig Drugs 10, 321-9.
- Dotiwala, F. and Lieberman, J. (2019) Curr Opin Immunol 60, 19-29.
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