GPRIN1 (E8A7B) Rabbit mAb #62990
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 135 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
GPRIN1 plays a role in the activation of nicotinic acetylcholine pathways by interacting with β2 subunits of nicotinic acetylcholine receptors (nAChRs) and other nAChR-interacting proteins, including G protein α and the G protein-activated K+ channel 1 (2). The observed neurite outgrowth of neurons in the CA3 and CA1 hippocampus is facilitated by interaction with the α7 subunit of nAChR, which is dependent on interaction with the G protein complex pathway (GPC), including Gαi/o (3,4).
Additionally, the interaction of GPRIN1 with the serotonin receptor 5-HT6 potentiates the 5-HT6R constitutive activation of the Gs-coupled receptors pathways, promoting neurite elongation and branching of striatal neurons. CDK5 interacts with the 5-HT6R C-terminal domain resulting in the phosphorylation of 5-HT6R at Ser350, increasing the affinity of GPRIN1 (5).
- Iida, N. and Kozasa, T. (2004) Methods Enzymol 390, 475-83.
- Kabbani, N. et al. (2007) Proc Natl Acad Sci U S A 104, 20570-5.
- Nordman, J.C. and Kabbani, N. (2012) J Cell Sci 125, 5502-13.
- Nordman, J.C. et al. (2014) J Neurochem 129, 649-62.
- Pujol, C.N. et al. (2020) Sci Signal 13, eaax9520. doi: 10.1126/scisignal.aax9520.
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