Granzyme A (E4M9S) Rabbit mAb #76135
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 28 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Granzyme A (E4M9S) Rabbit mAb recognizes endogenous levels of total Granzyme A protein. This antibody does not cross-react with human Granzyme B, H, K, or M proteins.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu115 of human Granzyme A protein.
Background
Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein (2). Granzyme A, or GrA, is a dominant mediator of toxicity in vitro, and is the most abundant protease in cytotoxic granules (3). Granzyme A cleaves substrate after Arg or Lys and can activate caspase-independent cell death pathways upon cleavage of the mitochondrial protein NDUFS3. Cleavage of NDUFS3 leads to reactive oxygen species (ROS) generation, which triggers translocation of the SET complex to the nucleus, where Granzyme A cleaves the SET complex and leads to the opening of DNA and DNA nicks (4-7).
- Trapani, J.A. (2001) Genome Biol 2, REVIEWS3014.
- Lord, S.J. et al. (2003) Immunol Rev 193, 31-8.
- Froelich, C.J. et al. (2009) Trends Immunol 30, 117-23.
- Martinvalet, D. et al. (2008) Cell 133, 681-92.
- Fan, Z. et al. (2003) Nat Immunol 4, 145-53.
- Beresford, P.J. et al. (1999) Immunity 10, 585-94.
- Zhang, D. et al. (2001) Proc Natl Acad Sci USA 98, 5746-51.
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