Granzyme K (E9I3O) Rabbit mAb #47465
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 17-27 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Granzyme K (E9I3O) Rabbit mAb recognizes endogenous levels of total Granzyme K protein. This antibody does not cross-react with human Granzyme A, B, H, or M proteins.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp196 of human Granzyme K protein.
Background
Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3).
Granzyme K has tryptase-like activity with a preference for cleaving its specific substrates after basic residues, arginine or lysine (4-7). Granzyme K is expressed by NK T cells, cytotoxic T cells, and NK cells (8,9). After perforin-mediated entry into a target cell, Granzyme K induces apoptosis via generation of reactive oxygen species, DNA damage, and BID-dependent mitochondrial damage (10-12).
Limited Uses
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