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R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GRAP (F9U7T) Rabbit mAb #51888

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  • WB
  • IP

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 25
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GRAP (F9U7T) Rabbit mAb recognizes endogenous levels of total GRAP protein. This antibody does not cross-react with GRB2 protein.

    Species Reactivity:

    Human, Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Pig

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GRAP protein.

    Background

    GRB2-related adaptor protein (GRAP) is a member of the GRB2 family of signaling adaptor proteins, and its expression is largely restricted to lymphoid tissues (1,2). Similar to the structure of other members of the GRB2 family of adaptor proteins, GRAP contains a central SH2 domain flanked on either side by SH3 domains that allow for it to directly associate with other signaling molecules (1). Research studies have suggested that, unlike GRB2, GRAP may hinder T cell effector functions by disrupting Ras/MAPK signaling downstream of the T cell receptor signaling complex (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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