GRASP55/GORASP2 (F8L6I) Rabbit mAb #48592

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	61
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

GRASP55/GORASP2 (F8L6I) Rabbit mAb recognizes endogenous levels of total GRASP55/GORASP2 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val425 of human GRASP55/GORASP2 protein.

Background

In unconventional protein secretion (UPS), cytoplasmic cargo proteins that do not have a signal peptide or a transmembrane domain are transported to the plasma membrane or extracellular matrix mainly to respond to cellular stresses (1,2). The Golgi reassembly-stacking protein 2 (GRASP55, also known as GORASP2) is essential to type III and type IV UPS (2,3). Studies show that mTORC1 phosphorylates GRASP55/GORASP2 and keeps it at the Golgi apparatus under non-stressed conditions. When cells are stressed, mTORC1 is inhibited, leading to dephosphorylation of GRASP55/GORASP2. Dephosphorylated GRASP55/GORASP2 relocates to autophagosomes and multivesicular bodies to promote UPS. In addition, Thr264 on GRASP55/GORASP2 was shown to seemingly play a key role in mTORC1-mediated phosphorylation of the protein (2).

Database Links

UniProt ID: Q9H8Y8

Entrez-Gene Id: 26003

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For Research Use Only. Not For Use In Diagnostic Procedures.

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