Confocal immunofluorescent analysis of SK-BR-3 cells (left, positive) and U-118 MG cells (right, negative) using GRB7 (E3W4G) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
|Immunofluorescence (Immunocytochemistry)||1:200 - 1:800|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Achieve higher quality immunofluorescent images using the efficient and cost-effective, pre-made reagents in our #12727 Immunofluorescence Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Recommended Fluorochrome-conjugated Anti-Rabbit secondary antibodies:
NOTE: Cells should be grown, treated, fixed and stained directly in multi-well plates, chamber slides or on coverslips.
Aspirate liquid, then cover cells to a depth of 2–3 mm with 4% formaldehyde diluted in 1X PBS.
NOTE: Formaldehyde is toxic, use only in a fume hood.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
posted November 2006
revised November 2013
Protocol Id: 24
GRB7 (E3W4G) Rabbit mAb recognizes endogenous levels of total GRB7 protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro87 of human GRB7 protein.
GRB7 belongs to the GRB7 family of adaptor proteins that mediate cell surface and cytosolic tyrosine kinases to downstream signaling pathways (1-3). The C-terminal SH2 domain of GRB7 has been shown to be important for its interaction with various upstream molecules such as ERBB receptor tyrosine kinases, FAK, EphB1, and SHC. The protein has an RA, CAM-BD, and PH domain located at the central region (GM region) of its sequences to mediate downstream Ras, PI3K/Akt, ERK, and Calmodulin signaling (3,4). Overexpression of GRB7 promotes cancer proliferation and migration, and disruption of GRB7 function inhibits its effect on cancer growth, invasion, and metastasis (3-5).
Explore pathways + proteins related to this product.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
ProLong is a registered trademark of Life Technologies Corporation.