GRK6 (D1A4) Rabbit mAb #5878
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
GRK6 (D1A4) Rabbit mAb recognizes endogenous levels of total GRK6 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu89 of human GRK6 protein.
Background
G protein-coupled Receptor Kinase 6 (GRK6) is one of 7 members of the GRK serine/threonine kinase subfamily, which are known primarily for their role in desensitizing activated G protein-coupled receptors (GPCRs) (1,2). GRKs function by phosphorylating serine/threonine residues in activated GPCRs. Upon phosphorylation these residues serve as binding sites for β-arrestin proteins, inhibiting re-activation of GPCRs by blocking their re-association with G proteins (3). There is evidence that GRKs can also modulate selected non-GPCR signaling pathways (2). For example, GRK6 has been shown to modulate the Wnt signaling pathway via phosphorylation of LRP6 (4), and the insulin-like growth factor signaling pathway (5). GRK6 may also play a role in immune system function. Investigators have found GRK6 expression is typically abundant in hematopoietic tumor cell lines, and a recent research study demonstrated that GRK6 suppression was selectively lethal for a number of myeloma tumor cell lines (6).
Limited Uses
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