GSR (E5L1A) Rabbit mAb #62448
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 56 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
Storage
Protocol
Specificity / Sensitivity
GSR (E5L1A) Rabbit mAb recognizes endogenous levels of total GSR protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly130 of human GSR protein.
Background
Glutathione reductase (GR), also known as glutathione-disulfide reductase (GSR), catalyzes the conversion of glutathione disulfide (GSSG) to reduced glutathione (GSH) to maintain GSH levels in the cell (1-3). GSR is one of the enzymes in mitochondria that remove excessive reactive oxygen species (ROS) and protect cells from oxidative stress (2). Studies show that AMPKα1 plays a role in the phosphorylation of GSR in colorectal cancer (CRC) cells upon glucose deprivation, increasing GSR enzymatic activity. Enhanced GSR activity supports high levels of reduced glutathione to maintain cellular redox homeostasis under stress conditions (4).
Limited Uses
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