Render Target: STATIC
Render Timestamp: 2024-11-05T10:42:54.655Z
Commit: 57f6e368eba1a427377652f2ad915d45d7f340a4
XML generation date: 2024-09-30 01:59:54.934
Product last modified at: 2024-09-30T08:01:04.305Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GSR (E5L1A) Rabbit mAb #62448

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 56
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GSR (E5L1A) Rabbit mAb recognizes endogenous levels of total GSR protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly130 of human GSR protein.

    Background

    Glutathione reductase (GR), also known as glutathione-disulfide reductase (GSR), catalyzes the conversion of glutathione disulfide (GSSG) to reduced glutathione (GSH) to maintain GSH levels in the cell (1-3). GSR is one of the enzymes in mitochondria that remove excessive reactive oxygen species (ROS) and protect cells from oxidative stress (2). Studies show that AMPKα1 plays a role in the phosphorylation of GSR in colorectal cancer (CRC) cells upon glucose deprivation, increasing GSR enzymatic activity. Enhanced GSR activity supports high levels of reduced glutathione to maintain cellular redox homeostasis under stress conditions (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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